Introduction to MF-SIM
In the field of fluorescence biological imaging, general interest is more and more turned towards video-rate 3D super-resolution microscopy. SIM is a good candidate for fast acquisition but until now, the acquisition of focal stacks was always performed sequentially. In this project, we aim to combine the fast-SIM set-up with a multi-focus detection scheme [4] and thus build a so-called “multi-focus structured illumination microscope” (MF-SIM).
MF-SIM set-up
Sketch of the MF-SIM set-up
The excitation arm is a clone of the fast-SIM system existing in the group [5, 6], with one major difference: this set-up is going to be a 3-beam SIM. The detection arm follows a multi-focus detection scheme, the important components being the multi-focus grating (MFG) which produces the desired multi-focus effect, the chromatic correction grating (CCG) and the multi-faceted prism which together correct for chromatic dispersion in the emitted fluorescence light [4].
Litterature MF-SIM
[4] Abrahamsson, S.; et al.
Fast multicolor 3D imaging using aberration-corrected multifocus microscopy
Nature Methods, Nature Publishing Group, 2013, 10, 60-63
[5] Förster, R.; et al.
Simple structured illumination microscope setup with high acquisition speed by using a spatial light modulator
Optics Express, 2014, 22 (17), 20663-20677
[6] Lu-Walther, H.; et al.
FastSIM: A practical implementation of fast structured illumination microscopy
Methods and Applications in Fluorescence, 2015, 3, 014001