This project, which is a part of the CRC 1278 PolyTarget (https://www.polytarget.uni-jena.de/), aimed at investigating optimized approaches to combine the mutual benefits of both imaging modalities: (fluorescence) light microscopy (LM) and electron microscopy (EM). The aim is to obtain molecule specific information by the LM measurement and to join it with the ultra-structure seen in the EM (Correlative light and electron microscopy). This shall be implemented by a custumized algorithm to enable an EM-guided deconvolution. The basic idea is to use the high-resolution information of the EM image to guide the deconvolution process of the LM images via a tailored regularisation. This will, for example, help the study of changes in the ultrastructural environment in response to nanoparticle uptake.
Contact: Fengjiao Ma