Dr. Daniela Täuber

Dr. Daniela Täuber

Heads the BioPOLIM Group working on anisotropy in microscopy imaging in Life and Materials Sciences

2DPOLIM

2-Dimensional Fluorescence Polarization Imaging (2DPOLIM) has been developed in the Lab of Ivan Scheblykin at Lund University (Camacho et al., Chemical Physics 2012; Camacho et al., Scientific Reports 2015). In Jena we have built a modified 2DPOLIM setup which combines electronic control of the linear polarization in excitation using a liquid crystal polarization rotator (Wang et al. 2023) with a fixed four-channel detection. The development of the setup and further work on application of 2DPOLIM to cells and tissue was funded (06/2002 - 05/2024) by the German Research Foundation (DFG proj. 439139881). The project work included complementary work using mid-IR photo-induced force microscopy (IR-PiFM / PiF-IR), see Joseph et al. Spectrochimica Acata A, 2024.

By recording fluorescence intensities under different excitation and detection polarization angles, 2DPOLIM provides full in-plane polarization information. Additionally photophysics in the sample can be investigated by evaluating relations between polarization parameters in excitation and in emission (Camacho, Täuber, Scheblykin, Advanced Materials 2019).

Our current work focuses on 2DPOLIM measurements to study the reorganization of the cytoskeletal protein F-actin in cells and tissue ex vivo. By combining these measurements with molecular dynamics simulations, we study the molecular orientation and photophysics of fluorophores associated with F-actin.

Involved people: Daniela Täuber, Yutong Wang, Yunhao Mei, Asad Hafeez, Shangjun Cheng.

Publications:

  • J. Joseph, L. Spantzel, M. Ali, D.M. Joseph, S. Unger, K. Reglinski, C. Krafft, A.-D. Müller, C. Eggeling, R. Heintzmann, M. Börsch, A.T. Press, D. Täuber: Nanoscale chemical characterization of secondary protein structure of F-Actin using mid-infrared photoinduced force microscopy (PiF-IR). Spectrochimica Acta part A: Molecular and Biomolecular Spectroscopy, 306, 123612, 2024. doi:10.1016/j.saa.2023.123612
  • S. Cheng, S. Gjeci, A. Rusevski, P. Then, E. Sunil, S. Adak, H.-D. Arndt, R. Heintzmann, A.T. Press, D. Täuber: F-Actin Fluorescence Staining Evaluated Using 2D Polarization Fluorescence Imaging (2DPOLIM), Structured Illumination Microscopy (SIM) and Fluorescence Lifetime Imaging (FLIM), 57th Winter Seminar - Biophysical Chemistry, Molecular Biology and Cybernetics of Cell Functions, Klosters, Switzerland, January 2024. doi:10.13140/RG.2.2.14239.33443
  • Y. Wang, A. Hafeez, M. Soltaninezhad, R. Heintzmann, D. Täuber: Fast excitation modulation via liquid crystal polarization rotator in 2D polarization fluorescence imaging (2D POLIM) and compensation of depolarization caused by dichroic mirrors, 56th Winter Seminar - Biophysical Chemistry, Molecular Biology and Cybernetics of Cell Functions, Klosters, Switzerland, January 2023. doi:10.13140/RG.2.2.35169.79204
  • D. Täuber, J. Shi, P. Babic, K.-J. Benecke, M. Bauer, R. Heintzmann, I.G. Scheblykin, and A.T. Press: Imaging aggregation of histologically stained F-actin ex vivo using the contrast in Förster resonance energy transfer obtained from 2D polarization fluorescence imaging (2D-POLIM), European Light Microscopy Initiative 2021, June 2021. doi:10.22443/rms.elmi2021.6