In structured illumination microscopy (SIM) high spatial frequencies of the sample (finer object structures) are down-modulated into the transfer band of the objective by applying a stripe shaped illumination pattern. Hence structured illumination improves the resolution. The research work, regarding SIM focuses on:

  • Multicolor SIM with up to three different excitation wavelengths
  • Fast SIM with a recording rate of up to 700 images per second
  • Multifocal SIM for simultaneous detection of several focal planes
  • Nonlinear SIM to further enhance the lateral resolution
  • Polarization coded SIMĀ  for improving the optical sectioning
  • Multiphoton SIM for achieving highest resolution deep inside strongly scattering tissue
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