In structured illumination microscopy (SIM) high spatial frequencies of the sample (finer object structures) are down-modulated into the transfer band of the objective by applying a stripe shaped illumination pattern. Hence structured illumination improves the resolution. The research work, regarding SIM focuses on:
- Multicolor SIM with up to three different excitation wavelengths
- Fast SIM with a recording rate of up to 700 images per second
- Multifocal SIM for simultaneous detection of several focal planes
- Nonlinear SIM to further enhance the lateral resolution
- Polarization coded SIM for improving the optical sectioning
- Multiphoton SIM for achieving highest resolution deep inside strongly scattering tissue