A second approach in order to enhance the resolution is direct stochastic optical reconstruction microscopy (dSTORM). In dSTORM a series of images is recorded. At each image only a small fraction (statistically determined) of all fluorophores is emitting light. Due to the sparse emission pattern, the emission centers can be determined with very high accuracy. The high resolution image is subsequently reconstructed by superposing all frames of the series.

Currently we aim for combining this method with automated multiple staining and image recording steps in order to develop a systematically working high resolution analysis tool.

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